New generation of bioinks shows promise for more sustainable 3D bioprinting.
Heidelberg researchers create biocompatible inks from algae
An international research team led by Prof. Eva Blasco at the Institute for Molecular Systems Engineering and Advanced Materials (IMSEAM) in Heidelberg University, has succeeded in manufacturing inks for printing complex biocompatible 3D microstructures from the raw materials extracted from algae.
According to the researchers, the microalgae-based materials could be used in future as the basis for implants or scaffolds for 3D cell cultures. Microalgae such as the diatom Odontella aurita and the green alga Tetraselmis striata are especially suitable as “biofactories” for the production of sustainable materials for 3D laser printing due to their high content in lipids and photoactive pigments.
Two-photon 3D laser printing offers particular advantages for manufacturing at the micro- and nanoscale. Owing to its remarkable resolution, it finds application in numerous fields including optics and photonics, microfluidics, and biomedicine. The process involves focusing a laser beam on a liquid, photoreactive resin. At the focal point, the laser light activates photoinitiators to trigger a chemical reaction, causing local solidification of the resin.
To date, petrochemical-based polymers have been mainly used as inks for this 3D laser printing process. However, these polymers contribute to the depletion of fossil fuels and the emission of greenhouse gases and can also contain toxic components, claims Blasco.
Fortunately, microalgae are well suited to the production of sustainable materials for 3D printing due to their rapid growth rate, CO2-fixation during cultivation and biocompatibility.
“Despite their advantages, microalgae have hardly been considered as raw materials for light-based 3D printing,” said Blasco in a press release. Her group conducts research at the interface of macromolecular chemistry, materials science and 3D nanofabrication.
For their experiments, the researchers selected the two species noted above –Odontella aurita and Tetraselmis striata – that contain particularly high levels of lipids in the form of triglycerides. The team extracted the triglycerides and functionalized them with acrylates to facilitate rapid curing under light irradiation. The photoactive green pigments present in the microalgae proved to be suitable as photoinitiators. When exposed to light, they trigger the chemical reaction that solidifies the ink into a three-dimensional structure.
“In this way we avoid using potentially toxic additives like the photoinitiators used in conventional inks,” explained first author Clara Vazquez-Martel, a doctoral candidate in Blasco’s research team at IMSEAM.
Using the new ink system, the researchers were able to produce different 3D microstructures with high precision, exhibiting complex features such as overhanging roofs and cavities. Using cell culture experiments, the researchers also investigated the biocompatibility of the microalgae-based inks. They prepared 3D microscaffolds on which the cells were cultured and demonstrated a survival rate of almost 100%.
“Our results open up new possibilities not only for more sustainable 3D printing with light, but also for life science applications – from 3D cell cultures to biocompatible implants,” Blasco said.
The research is published in the journal Advanced Materials.
